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@sbpressurewashing: Her reaction when I got done #pressurewashing #satisfying #cleaning #asmr #sbpressurewashing #asmrvideo #satisfyingvideo #powerwashing #fypシ #fyp #viral #viralvideo #homeowners #cleaningtiktok #cleanup

SB Pressure Washing
SB Pressure Washing
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Region: US
Friday 14 July 2023 14:22:55 GMT
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Comments

no_sarahtonin
sarah ❤️‍🔥 :
Finally someone appreciative
2023-07-14 18:24:07
303
its_a_female_human
Carys :
ugh we need a canadian snow blower person that does this
2023-07-16 22:46:36
66
excommunicadios
Excommunicados :
Can you provide any tips on how to close deals with potential customers? Started last month and haven’t had a yes yet.
2023-07-14 14:28:11
12
truecrimewithjordy
Jordy | True Crime 🚔 :
Idk why at first it already looked clean lol
2026-02-19 01:55:31
0
arielleedaraa
Arielleedaraa<333 :
Wish u were around when my grandma was alive she use to scrub with a old scrub brush
2023-09-11 03:42:56
5
yelnay21
Yelnay_Gonzalo :
No tips at least?? I would tii at least.. u are do niceee
2023-09-08 23:01:12
0
natedog1821
nateschoeneman :
third
2023-07-14 14:39:30
2
wayoutwest1984
2Legit2Quit :
What’s a good way to clean dirty concrete without pressure washing? Former owners over-washed the back patio, but now it’s very stained and dirty.
2023-07-30 13:25:43
0
anywherebuthere94
anywhere but here945 :
Do you get the water from home owner or have a tank at back of pickup ?
2024-03-04 04:43:00
0
navyman114
NAVY :
You should do a video backwards
2023-07-14 16:20:27
2
____asher___
____Asher___ :
Wonderful
2023-07-16 09:27:10
2
jeffrobinson4
ShadowBoy :
Did it get rid of the car drips ?
2023-08-01 14:13:19
0
desmond_gab
D_Gab :
How much it cost for the surface cleaner? Tyia
2023-07-27 02:49:55
0
debra77779
🥀Debra 🥀 :
Awesome job 👏👏👏♥️
2023-09-18 12:13:17
0
lalamimiisa
lalamimiisa :
o que você faz ,deixa as pessoas feliz tráz a alegria ,parabéns
2023-07-21 02:10:28
0
hensu_boy_r.j
Mr.Perfect🤞🫶👑 :
🥰
2025-05-25 00:56:02
1
cathyvill
Catherine Ocampo :
🥰🥰🥰🥰🥰
2023-07-14 23:02:59
1
langgalai81
Langga Lai :
🥰🥰🥰🥰
2023-08-30 05:10:13
1
valentinasalinas29
Debra Jean Garza :
🙏🙏🙏
2025-02-27 14:06:39
0
peaceful239
Liz :
🙏🙏🙏
2025-02-18 19:01:05
0
corazonrox
corazonrox :
👍
2023-09-17 08:33:59
0
peaceful239
Liz :
❤️❤️❤️
2025-02-18 19:01:05
0
kellywritt
Kelly Writt Cooper :
I would feel guilty having someone do all that work for free. Does anyone tip?
2023-09-19 12:44:16
2
je1974
JE :
U used a circular brush to clean the driveway… i wonder how powerful it is… and about energy consumption…
2023-07-15 13:38:16
0
To see more videos from user @sbpressurewashing, please go to the Tikwm homepage.

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Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks:
Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks: "Does this DNA match my guide, and is there an appropriate PAM next to it?" 5. Then Cas9 cuts both DNA strands Once the target is recognized, Cas9 creates a double-strand break. And this is where the actual editing happens. The cell has to repair the broken DNA. Two major repair pathways are relevant: NHEJ — Non-Homologous End Joining The cell essentially reconnects the broken ends. It's relatively error-prone and can introduce small insertions or deletions (indels). This can disrupt a gene: functional gene → Cas9 cut → indel → disrupted gene This is commonly used for gene knockout. HDR — Homology-Directed Repair If researchers provide a suitable DNA template, the cell can use that template during repair. This allows much more precise changes, such as: A → G or insertion of a specific DNA sequence. The conceptual chain You can remember the whole thing as: Natural system Virus → CRISPR stores viral DNA fragment → guide RNA → Cas9 → viral DNA cut ↓ Human engineering Choose target DNA → design guide RNA → Cas9 → target DNA cut → cell repairs it → genetic change It's slightly more precise to say CRISPR is a bacterial/archaeal adaptive immune system, rather than "CRISPR-Cas9 is gene editing." CRISPR refers primarily to the characteristic genomic repeat/spacer system. Cas proteins are the associated molecular machinery. Cas9 is one particular CRISPR-associated nuclease. And CRISPR-Cas9 gene editing is the human-developed application of that naturally occurring machinery. There are also many other CRISPR systems—Cas12, Cas13, etc.—which have different properties and can target DNA or RNA.


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Wednesday morning roll call completed 📣 All pooches present and correct ☑️ #dog #doggo #dogsofinstagram #dogsvideo
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