@mrzokeer: Consigue las HOCES DE HIELO Facil y Rapido en GROUNDED 2 #grounded2 #grounded

MrZokeer

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Sunday 03 August 2025 17:50:00 GMT

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A X :

esto no tiene nada que ver con el video pero ya estoy desesperado. el juego me saca constantemente, como 7 veces al día o mas y ta es muy desesperante. alguien sabe que puedo hacer? juego en la series S

2025-08-03 21:16:25

123

kest3l_cv22 :

Pero lo intenté pero siempre me destruyen la arma, saben pq?

2025-08-03 23:02:03

34

Arnauff :

Se me ha bugeado i no me salen las marcas. Sabes como solucinarlo?

2025-08-03 18:19:02

14

AlbertBc :

nuevo seguidor !!!

2025-08-07 17:22:22

1

《× DΛV1ÐTRҒ² ×》 :

yo ni sé q grounded tengo

2025-08-04 00:56:04

1

tiranierP 💥 :

pero me aparece un barra de vida y si se cava no la dan :/

2025-08-04 02:18:23

6

GoatOfSatan :

Lo acabo de hacer, pero si atacan las dagas, lo que hice fue usar el scorching orb, y desde arriba del foco les disparaba para llamar su atención y dejaran de atacar las dagas, y así lo logré.

2025-08-05 05:25:32

15

Sotangor23 :

como conseguir que no vaya a 15fps?? hahaha mira que lo he tocado todo....

2025-08-09 10:58:27

1

𝔇𝔞𝔪𝔦𝔞𝔫 𝔪𝔞𝔯𝔣𝔲𝔩𝔩 :

Que pasa si eh muerto y al volver me dice que las repare y no me deja?

2025-08-06 01:13:18

1

Emmanuel :

por que al principio ceia los graficos bien y ahora los veo muy como del graunded 1 en xbox one?

2025-08-08 20:53:54

0

Charly Quintero :

Cada vez que muero mi mochila queda fuera del mapa jajaja se arregla de alguna manera? A mis amigos en la misma partida no les pasa

2025-08-04 19:52:07

0

chamoy :

cómo se quita el bug de la abeja

2025-08-03 23:09:23

0

robjuu :

a mi se me bugueo la hormiga y no puedo tener otra, no puedo seguir la historia o avanzar a otras zonas

2025-08-03 22:48:01

0

ERU ILUVATAR :

Esa camara rapida no ayuda nada amigo

2025-08-08 21:09:02

2

gusi :

desactiven el cromático para más fluidez😅

2025-08-10 18:45:46

3

max0514 :

es mejor estar en la punta del tenedor

2025-08-09 20:28:06

0

Pedro Cortes Rodrigu :

me han matado en la superficie y mi mochila aparece bajo tierra, saben que puedo hacer? juego en Xbox gracias

2025-08-03 21:49:42

0

Santiago Vazquez :

en la zona gélida justo cerca del lugar donde se encuentran las hoses hay una bugueada

2025-08-06 00:56:19

0

chamoy :

con que armadura fuiste?

2025-08-13 04:52:23

1

chamoy :

con que armadura fuiste?

2025-08-03 19:29:05

2

Israel :

oye bro, puedes hacer un video mostrandolas más a detalle, no se que tan chetas estan

2025-08-04 00:06:37

2

Star_Cat :

A mí se me crashea en esa zona

2025-08-05 11:30:04

2

adaa :

la primera vez no lo hice bn y no me las dieron pero quiero hacerlo otra vez y me sale reparar como le hago?

2025-08-27 18:13:33

1

alex18 :

Entonces solo quedo mirando que estén ahí xd ?

2025-08-03 19:32:32

2

Palomitas :

dónde esta el cuarpo

2025-08-03 20:59:41

1

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Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks:

Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks: "Does this DNA match my guide, and is there an appropriate PAM next to it?" 5. Then Cas9 cuts both DNA strands Once the target is recognized, Cas9 creates a double-strand break. And this is where the actual editing happens. The cell has to repair the broken DNA. Two major repair pathways are relevant: NHEJ — Non-Homologous End Joining The cell essentially reconnects the broken ends. It's relatively error-prone and can introduce small insertions or deletions (indels). This can disrupt a gene: functional gene → Cas9 cut → indel → disrupted gene This is commonly used for gene knockout. HDR — Homology-Directed Repair If researchers provide a suitable DNA template, the cell can use that template during repair. This allows much more precise changes, such as: A → G or insertion of a specific DNA sequence. The conceptual chain You can remember the whole thing as: Natural system Virus → CRISPR stores viral DNA fragment → guide RNA → Cas9 → viral DNA cut ↓ Human engineering Choose target DNA → design guide RNA → Cas9 → target DNA cut → cell repairs it → genetic change It's slightly more precise to say CRISPR is a bacterial/archaeal adaptive immune system, rather than "CRISPR-Cas9 is gene editing." CRISPR refers primarily to the characteristic genomic repeat/spacer system. Cas proteins are the associated molecular machinery. Cas9 is one particular CRISPR-associated nuclease. And CRISPR-Cas9 gene editing is the human-developed application of that naturally occurring machinery. There are also many other CRISPR systems—Cas12, Cas13, etc.—which have different properties and can target DNA or RNA.

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