@physiotj: Most guys don’t realize there’s a normal course of testing for suspected bacterial prostatitis — 🧪 Step 1: Urinalysis This looks for bacteria or inflammation in a standard urine sample. Quick, simple, but not always specific. 🧪 Step 2: Two-Glass Test You give a sample before and after prostate massage. If bacteria show up more in the post-massage sample, it suggests the prostate is involved. 🧪 Step 3: Next-Gen Sequencing (NGS) This test can detect tiny amounts of bacterial DNA, but here’s the catch: • It’s not very sensitive for typical prostate pathogens • It can detect harmless bacteria and lead to over-treatment • That’s why most urologists don’t recommend it as routine care Bottom line: “Negative” tests don’t always equal “mystery infection.” They often mean it’s time to look beyond bacteria — pelvic floor dysfunction and nervous system sensitization due to chronic pain/inflammation could be the reason the antibiotics just aren’t getting the job done! If this sounds like you, talk to a pelvic floor physical therapist today! #pelvichealth #pelvicpain #prostatehealth #pelvicfloorphysicaltherapy