@opticavisaoperfeita: Influenciador me pediu 2.000 pra fazer Marketing! E meu amigo flanelinha me cobrou 100,00 #virlvideo #trabalho #tiktok

@Opticavisaoperfeita

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Thursday 09 April 2026 17:20:17 GMT

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🙌 :

divulgação ficou maneiro tudo ao vivo sem mimimi chama

2026-04-14 02:15:02

3

Ruanito Oliveira :

Esse caba é desenrolado 👍

2026-05-02 15:15:39

1

ws_sntts :

O cabra saiu até bem viu tudo sem roteiro isso é ótimo as melhores divulgações e sem roteiro nenhum

2026-04-10 18:51:58

2

GIAN👨🏻‍🏭🔥K.B.LO F🔥G🔥ALTO :

o caba é bom na divulgação, gostei simplicidade em alto nível

2026-04-10 14:34:00

3

nt vagabundo :

Já passei no centro de aju e sigo no TikTok, ele é muito gente boa

2026-06-08 02:05:00

1

Levi Santos Rodrigues :

dado flanelinha

2026-05-24 20:47:54

1

juniorvideomakker :

👏🏾👏🏾👏🏾 mesmo sem edição o vídeo ficou legal

2026-05-10 01:03:53

1

negrão :

mandou vê 👏👏👏👏👏 show de bola.

2026-06-09 22:17:16

1

Gilberto Vieira :

valeu

2026-05-26 20:00:03

1

💯 :

“Quer mais o quê?”KKKKKKKK

2026-04-23 03:09:20

1

breno :

Boa

2026-04-09 23:11:15

2

clecio silva :

conheço esse rapaz a muitos anos

2026-04-23 23:44:24

1

Ezequiel Dos Santos Santos :

Ai sim em show de bola 👏👏👏👏

2026-04-11 01:05:40

0

jirlan Vieira Cabral :

top mesmo q Deus abençoe a todos

2026-04-10 05:56:43

2

kayky_menezes_020 :

E ficou muito bom mesmo 🙏🏻

2026-04-09 23:54:30

2

user68168144085261 :

2026-04-10 12:38:45

1

hiagoferreira93 :

👏👏👏

2026-05-12 00:54:40

1

Rose Landim273 :

@Wellington Freitas F

2026-04-25 13:12:57

1

Cida :

👏

2026-04-14 14:54:52

1

Gustavo Santos Souza :

👏👏👏👏👏👏👏

2026-04-10 23:41:59

1

Ivan Mauricio :

👏👏👏👏👏👏👏

2026-04-11 18:59:01

1

Jaqueline rotinas :

👏👏👏👏👏

2026-04-11 15:57:30

1

Danzinho :

🔥🔥🔥

2026-04-09 19:23:18

1

Israel Juniooo. :

👏👏👏

2026-04-10 11:09:22

1

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Other Videos

COPS REGRET ARRESTING THIS ARMY VETERAN #cop #copsoftiktok #police #Viral #us

COPS REGRET ARRESTING THIS ARMY VETERAN #cop #copsoftiktok #police #Viral #us

Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks:

Crispr Cas9 is the defense mechanism of bacteria. #pharmacology #biology #crispr #genetics #iqmaxx Aw the video is a bit laggy ty capcut and tiktok 1. What does CRISPR actually stand for? CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats. It's a particular DNA sequence architecture found in bacteria and archaea. A CRISPR locus roughly looks like: Repeat – spacer – repeat – spacer – repeat – spacer... The spacers are fragments of DNA that the bacterium has acquired from viruses that previously infected it. So CRISPR is essentially a form of molecular immune memory. 2. Where does Cas9 come in? Cas9 = CRISPR-associated protein 9. Cas proteins are proteins associated with CRISPR systems. Cas9 is an endonuclease, meaning it can cut DNA. In the natural bacterial system: A bacterium gets infected by a virus. The bacterium incorporates a small piece of the viral DNA into its CRISPR locus. That DNA can later be transcribed into a guide RNA. The guide RNA associates with Cas9. If the same virus attacks again, the guide RNA helps Cas9 recognize the matching viral DNA. Cas9 cuts the viral DNA, helping destroy the infection. So conceptually: previous infection → DNA memory → guide RNA → Cas9 → matching viral DNA gets cut 3. Humans basically hijacked this system This is the important part. Scientists realized: If we can program the RNA that directs Cas9, we can tell Cas9 to cut essentially any DNA sequence we want. Instead of a bacterial spacer derived from a virus, we provide a designed guide sequence complementary to the DNA sequence we're interested in. So: designed guide RNA + Cas9 → specific DNA sequence recognized → DNA cut That's the basis of CRISPR-Cas9 gene editing. CRISPR itself isn't synonymous with gene editing. It's the underlying biological system/toolset that humans adapted for editing. 4. How does Cas9 know where to cut? There are two major recognition components: Guide RNA (gRNA) Provides sequence specificity by base-pairing with the target DNA. PAM (Protospacer Adjacent Motif) A short DNA sequence immediately next to the target that Cas9 requires for recognition. For the commonly used Streptococcus pyogenes Cas9 (SpCas9), the canonical PAM is: 5'-NGG-3' where N = any nucleotide. Cas9 therefore isn't simply looking for the guide sequence. It effectively asks: "Does this DNA match my guide, and is there an appropriate PAM next to it?" 5. Then Cas9 cuts both DNA strands Once the target is recognized, Cas9 creates a double-strand break. And this is where the actual editing happens. The cell has to repair the broken DNA. Two major repair pathways are relevant: NHEJ — Non-Homologous End Joining The cell essentially reconnects the broken ends. It's relatively error-prone and can introduce small insertions or deletions (indels). This can disrupt a gene: functional gene → Cas9 cut → indel → disrupted gene This is commonly used for gene knockout. HDR — Homology-Directed Repair If researchers provide a suitable DNA template, the cell can use that template during repair. This allows much more precise changes, such as: A → G or insertion of a specific DNA sequence. The conceptual chain You can remember the whole thing as: Natural system Virus → CRISPR stores viral DNA fragment → guide RNA → Cas9 → viral DNA cut ↓ Human engineering Choose target DNA → design guide RNA → Cas9 → target DNA cut → cell repairs it → genetic change It's slightly more precise to say CRISPR is a bacterial/archaeal adaptive immune system, rather than "CRISPR-Cas9 is gene editing." CRISPR refers primarily to the characteristic genomic repeat/spacer system. Cas proteins are the associated molecular machinery. Cas9 is one particular CRISPR-associated nuclease. And CRISPR-Cas9 gene editing is the human-developed application of that naturally occurring machinery. There are also many other CRISPR systems—Cas12, Cas13, etc.—which have different properties and can target DNA or RNA.

@deepstorycast #deepstory #deepstoryclipfy #clipfyleague

@deepstorycast #deepstory #deepstoryclipfy #clipfyleague

Bản thân em 1% là ngọt ngào. 99% còn lại là trẻ trâu =)))) #xuhuongtiktok #xuhuonggggggggggggggggg

Bản thân em 1% là ngọt ngào. 99% còn lại là trẻ trâu =)))) #xuhuongtiktok #xuhuonggggggggggggggggg

@deepstorycast #clipfyleague #deepstoryclipfy #deepstory

@deepstorycast #clipfyleague #deepstoryclipfy #deepstory

#duet with @user2208326653741

#duet with @user2208326653741

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