If you mean the Mona Lisa (the famous painting), the person depicted is generally believed to be female.
Most historians identify her as Lisa Gherardini, an Italian woman from Florence who was painted by Leonardo da Vinci around 1503.
There have been theories suggesting the painting could contain elements of Leonardo himself or have symbolic meanings, but there is no strong evidence that the subject was male. The widely accepted view is that the Mona Lisa portrays a woman.